Lymphangiogenesis in Hodgkin Lymphoma and in Indirectly Related Conditions – A Review

Article Information

Daniel Benharroch1*, Isebrand Prinsloo2, Jacob Gopas3, Benzion Samueli1

1Soroka Medical Center and FOHS, Ben Gurion University of the Negev, Israel

2Deceased and buried at Beer-Sheva, Israel

3Head of the Shraga Segal Department of Immunology, Microbiology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev, and Department of Oncology, Soroka Univ. Med. Centre, Beer-Sheva, Israel

*Corresponding Author: Dr. Daniel Benharroch, Soroka Medical Center and FOHS, Ben Gurion University of the Negev, Israel

Received: 18 March 2020; Accepted: 01 April 2020; Published: 03 April 2020

Citation: Daniel Benharroch, Isebrand Prinsloo, Jacob Gopas, Benzion Samueli. Lymphangiogenesis in Hodgkin Lymphoma and in Indirectly Related Conditions – A Review. Journal of Cancer Science and Clinical Therapeutics 4 (2020): 078-085.

Share at Facebook


The significance of lymphangiogenesis in malignant tumors and specifically in classic Hodgkin lymphoma has not been properly evaluated, in contrast with that of angiogenesis in these neoplasms. In the study reviewed herein, the relevance of lymphatic vessel proliferation was explored in 19 cases of classic Hodgkin lymphoma stained with the D2-40 (anti-podoplanin) antibody. In each case, three lymphatic vessel hot spots were analyzed twice. Of the 57 hot spots thus investigated, 15 were chosen at random for image acquisition and analysis and microvessel counting. The mean perimeter, major axis length, surface area and complexity factor for each hot spot were established by morphometry, and associations with clinical and laboratory traits of classic Hodgkin lymphoma were identified. No relationships were found with the clinical features or immunostaining for typical markers of this lymphoma. However, significant inverse relations were found with BAX, pRb and IκB-α expression in tumor cells, genes believed to play roles in apoptosis in this lymphoma. The mean lymphatic major axis length was inversely correlated with the complexity factor. Clinicopathological associations were further obtained for the expression of BAX, pRb, and IκB-α in a large cohort of classic Hodgkin lymphoma patients that was previously published. Since the issue was poorly explored at this point, we have reviewed the significance of lymphangiogenesis in indirectly associated conditions.


Lymphatic vessels; Complexity factor; Hodgkin lymphoma; pRb; IκB-α; BAX

Lymphatic vessels articles, Complexity factor articles, Hodgkin lymphoma articles, pRb articles, I?B-? articles, BAX articles

Lymphatic vessels articles Lymphatic vessels Research articles Lymphatic vessels review articles Lymphatic vessels PubMed articles Lymphatic vessels PubMed Central articles Lymphatic vessels 2023 articles Lymphatic vessels 2024 articles Lymphatic vessels Scopus articles Lymphatic vessels impact factor journals Lymphatic vessels Scopus journals Lymphatic vessels PubMed journals Lymphatic vessels medical journals Lymphatic vessels free journals Lymphatic vessels best journals Lymphatic vessels top journals Lymphatic vessels free medical journals Lymphatic vessels famous journals Lymphatic vessels Google Scholar indexed journals Complexity factor articles Complexity factor Research articles Complexity factor review articles Complexity factor PubMed articles Complexity factor PubMed Central articles Complexity factor 2023 articles Complexity factor 2024 articles Complexity factor Scopus articles Complexity factor impact factor journals Complexity factor Scopus journals Complexity factor PubMed journals Complexity factor medical journals Complexity factor free journals Complexity factor best journals Complexity factor top journals Complexity factor free medical journals Complexity factor famous journals Complexity factor Google Scholar indexed journals Hodgkin lymphoma articles Hodgkin lymphoma Research articles Hodgkin lymphoma review articles Hodgkin lymphoma PubMed articles Hodgkin lymphoma PubMed Central articles Hodgkin lymphoma 2023 articles Hodgkin lymphoma 2024 articles Hodgkin lymphoma Scopus articles Hodgkin lymphoma impact factor journals Hodgkin lymphoma Scopus journals Hodgkin lymphoma PubMed journals Hodgkin lymphoma medical journals Hodgkin lymphoma free journals Hodgkin lymphoma best journals Hodgkin lymphoma top journals Hodgkin lymphoma free medical journals Hodgkin lymphoma famous journals Hodgkin lymphoma Google Scholar indexed journals pRb articles pRb Research articles pRb review articles pRb PubMed articles pRb PubMed Central articles pRb 2023 articles pRb 2024 articles pRb Scopus articles pRb impact factor journals pRb Scopus journals pRb PubMed journals pRb medical journals pRb free journals pRb best journals pRb top journals pRb free medical journals pRb famous journals pRb Google Scholar indexed journals IκB-α articles IκB-α Research articles IκB-α review articles IκB-α PubMed articles IκB-α PubMed Central articles IκB-α 2023 articles IκB-α 2024 articles IκB-α Scopus articles IκB-α impact factor journals IκB-α Scopus journals IκB-α PubMed journals IκB-α medical journals IκB-α free journals IκB-α best journals IκB-α top journals IκB-α free medical journals IκB-α famous journals IκB-α Google Scholar indexed journals BAX articles BAX Research articles BAX review articles BAX PubMed articles BAX PubMed Central articles BAX 2023 articles BAX 2024 articles BAX Scopus articles BAX impact factor journals BAX Scopus journals BAX PubMed journals BAX medical journals BAX free journals BAX best journals BAX top journals BAX free medical journals BAX famous journals BAX Google Scholar indexed journals lymphangiogenesis articles lymphangiogenesis Research articles lymphangiogenesis review articles lymphangiogenesis PubMed articles lymphangiogenesis PubMed Central articles lymphangiogenesis 2023 articles lymphangiogenesis 2024 articles lymphangiogenesis Scopus articles lymphangiogenesis impact factor journals lymphangiogenesis Scopus journals lymphangiogenesis PubMed journals lymphangiogenesis medical journals lymphangiogenesis free journals lymphangiogenesis best journals lymphangiogenesis top journals lymphangiogenesis free medical journals lymphangiogenesis famous journals lymphangiogenesis Google Scholar indexed journals immunotherapy articles immunotherapy Research articles immunotherapy review articles immunotherapy PubMed articles immunotherapy PubMed Central articles immunotherapy 2023 articles immunotherapy 2024 articles immunotherapy Scopus articles immunotherapy impact factor journals immunotherapy Scopus journals immunotherapy PubMed journals immunotherapy medical journals immunotherapy free journals immunotherapy best journals immunotherapy top journals immunotherapy free medical journals immunotherapy famous journals immunotherapy Google Scholar indexed journals biological therapy articles biological therapy Research articles biological therapy review articles biological therapy PubMed articles biological therapy PubMed Central articles biological therapy 2023 articles biological therapy 2024 articles biological therapy Scopus articles biological therapy impact factor journals biological therapy Scopus journals biological therapy PubMed journals biological therapy medical journals biological therapy free journals biological therapy best journals biological therapy top journals biological therapy free medical journals biological therapy famous journals biological therapy Google Scholar indexed journals angiogenesis articles angiogenesis Research articles angiogenesis review articles angiogenesis PubMed articles angiogenesis PubMed Central articles angiogenesis 2023 articles angiogenesis 2024 articles angiogenesis Scopus articles angiogenesis impact factor journals angiogenesis Scopus journals angiogenesis PubMed journals angiogenesis medical journals angiogenesis free journals angiogenesis best journals angiogenesis top journals angiogenesis free medical journals angiogenesis famous journals angiogenesis Google Scholar indexed journals

Article Details

1. Introduction

Recently, it has been suggested that lymphatic vessel proliferation (lymphangiogenesis - LAG) may provide a better target for immunotherapy and biological therapy than angiogenesis, where such therapies have mainly failed [1-4]. Classic Hodgkin lymphoma (cHL) is a primary malignant neoplasm of the immune system. For practical purposes, it almost exclusively involves lymph nodes (LNs) at diagnosis. Whereas LAG in the LNs has been detected mainly in metastatic tumors, LAG has occasionally been described in primary malignant lymphomas [5-7]. Angiogenesis has rarely been detected in cHL. Such an unusual description has been investigated by morphometric methods, microvessel density or the hot spot technique [8]. Lymphangiogenesis and angiogenesis have been explored by evaluating the expression of VEGF and variants in cHL [9].

We have previously atempted to clarify the features of LAG in cHL by using a morphometric method and looking for clinicopathological correlations [10]. A review of the previously published work on this topic is presented herein. The possible relevance of LAG is investigated, and when highlighted, a search is carried out to demonstrate the genes and growth factors associated with the role of LAG in cHL in an expanded population of cHL patients. Since studies on LAG in lymphoma are scarce, we conclude the present paper with a review of the literature published recently on LAG in conditions related directly or indirectly to lymphoma.

2. Methods

For the original study on LAG in cHL, nineteen cases of mixed cellularity cHL, primarily involving the LNs, were selected from our archives of formalin-fixed, paraffin-embedded tissue samples. Five-micron-thick sections were submitted for immunohistochemistry (IHC) (avidin-biotin complex method) with the D2-40 antibody (1:10, Dako to identify lymphatic vessels (LVs) and an anti-CD34 antibody to identify the blood vessel (BV) endothelium. For each case, three lymphatic vessel hot spots were analyzed (57 hot spots in total). The hot spots were imaged at 200X magnification and then printed with black ink on white paper. The vessels were underlined in the images: the LVs were traced in brown, and the BV endothelium was traced in red.

Fifteen of the 57 hot spots, which were selected at random, were subjected to morphometry twice. For each case, mean LV perimeter, major axis length, surface area and mean (shape) complexity factor analyses used the microvessel count and image analysis approaches described by Korkopoulou et al. [8]. The complexity factor was computed as equal to 5π*area/perimeter2. The above parameters were correlated with clinical characteristics and were also related to CD30, EBV/LMP1, EBER, CD20, p53, MDM-2, pRb, BAX and IκB-α expression and to the apoptotic index [11]. In 10 distinct sections of the hot spots, double staining of LVs was performed with D2-40 (counterstained brown with a DAB substrate) and an anti-CD34 antibody (counterstained red with an AEC substrate). For this analysis, we used the method reported by Kyras et al. [12]. Statistical analysis employed the Mann-Whitney test for nonparametric values and Spearman's analysis for nonparametric correlations. Finally, we carried out a review of the recent (2014-2019) relevant literature on LAG and lymphomas, but included also conditions akin to lymphomas.

3. Results

3.1 Lymphangiogenesis in cHL

No statistically significant associations were found between the four lymphatic parameters and clinical characteristics. Statistically significant inverse relationships were obtained between the LV parameter mean lymphatic area and mean complexity factor and pRb expression. BAX was inversely and significantly linked with the mean surface area and the mean LV length. IκB-α expression was inversely and significantly related to the mean LV perimeter . Of all the LV parameters, only the LV major axis length was inversely correlated with the shape factor, and this correlation was significant (r=-.6; p=.03). The only significant association of the mean lymphatic perimeter was an inverse relationship with IκB-α expression (p=.015). A direct and significant relation was found between the shape factor and pRb expression [10].

3.2 Relevance of BAX, pRb and IκB-α expression in an expanded (n=178) cHL cohort

As these genes were shown to present significant relations with LAG in cHL, confirmation in a larger population was deemed necessary. BAX was inversely linked with bulky cHL (p=.040). No other significant clinical associations were found in this large cohort. pRb expression was inversely related to the expression of a sialylated CD15 antigen. The relationships between BAX expression and the biological markers of cHL, included direct links with MCL1, p53 and MDM-2 expression, and inverse links with BAK and BCL-2 expression [10].

A significant inverse association between IκB-α and LMP1 of EBV was demonstrated. Kaplan-Meier analysis showed no significant difference in overall survival among patients stratified by BAX expression. In addition, there was no relationship between IκB-α expression and overall survival in cHL (data not shown).

© 2016-2024, Copyrights Fortune Journals. All Rights Reserved