The Gene Mutation and Drug Resistant of Mycobacterium tuberculosis in Patients of Chongqing

Author(s): Yishu Tang, Peiyang Song, Huiting Su

Objective: To detect the infection and drug resistance of Mycobacterium tuberculosis (MTB) in Chongqing and provide a scientific basis for the prevention and treatment of tuberculosis.

Methods: DNA was collected from all new suspected patients in Chongqing from January 2014 to September 2017, Genechip technology was used to identify Mtb strain. Genechip technology detects mutations in the ropB gene (associated with resistance to rifampicin) at locus 511, locus 513, locus 516 locus 526, locus 531 or locus 533. Genechip technology was also used to detect mutations in the KatG gene and inhA gene?

Results: Genechip revealed that the Mtb infected male accounted for 73.98% and female accounted for 26.12%.The total drug resistance rate of rifampicin and isoniazid were 11.2% (122/110771). Genechip revealed that ropB gene of 72 strains were mutation. The highest mutation site was 531 (TCG) locus (37.5%, 27/72). The people with katG and inhA gene mutation were 50 patients. The most common mutation site was 315 (AGC) locus. The cavity, history of treatment, and irregular medication were the risk factor of drug-resistant Mtb.

Conclusion: Our report demonstrated the infected ratio and the drug resistant types of Mtb in Chongqing district. We should strengthen health management and provide psychosocial support, in order to reduce the risk of drugresistant Mtb