Development and Evaluation of Nutraceutical products from Soybean, Sorghum and Basil leaf using Response Surface Methodology
Author(s): Abiodun Olapade, Uchenna Umeohia
A central composite orthogonal experimental design (CCOD) was used to develop a nutraceutical product from soy protein isolate, sorghum pyrodextrin and basil instant soluble powder using their levels incorporation as the independent factors while functional, and antinutritional parameters served as the responses. The haematological and biochemical parameters of albino wistar rat fed with the preferred nutraceutical product were determined. Sensory profiling by 30 panelists using a 9-point hedonic scale was conducted. The response surface analysis of the dependent variables of the products revealed both quadratic and linear fitted effects (P<0.05).The developed nutraceutical product had water absorption capacity, 1.69g/g, oil absorption capacity, 0.78g/cm3 bulk density, 6.82 pH, 1.40mg/g trypsin inhibitor, 0.73mg/100g phytate, 0.54mg/100g tannin, and 2.15HU/mg haemagglutinins. The nutraceutical product boosted the homopeitic activities, reduced the fasting blood glucose and cholesterol of the experimental rats, while biochemical analysis indicated no toxicological implication. A regression graph of the observed and predicted physicochemical properties of the product showed a highly fitted model of R2 = 0.999. Consumer acceptability tests showed that the developed nutraceutical product was accepted by the panelists.