Comparison of Bis-Plus-D and API 20 Strep for the Identification of Streptococci in the Laboratory of the University Hospital of Befelatanana Antananarivo Madagascar

Author(s): Zafindrasoa Domoina Rakotovao-Ravahatra, Jimmy Anders Antilahy, Joely Nirina Rakotovao-Ravahatra, Andriamiadana Luc Rakotovao

Background: In medical analysis laboratories, techniques for identifying bacteria are currently becoming more and more numerous. The objective of this study is to compare the Bis-Plus-D and the API 20 Strep for the identification of streptococci

Methods: During the study period, 22 Gram-positive coci isolates were identified using Api 20 Strep and Bis-Plus-D.

Results: During the study period, 22 isolates of streptococci were identified simultaneously using Bis-Plus-D and Api 20 Strep. The streptococci identified were represented by Streptococcus anginosus, Streptococcus agalactiae, Streptococcus uberis, Enterococcus columbae, Streptococcus pyogenes, Streptococcus constellatus, Enterococcus durans Enterococcus casseliflavus, Enterococcus faecalis and Enterococcus faecium. This study showed 50% of discordant results between Bis-Plus-D and Api 20 Strep. Regarding the bacterial identification qualities, they were all excellent (100%) for Api 20 Strep. For Bis-Plus-D, the identification qualities were excellent for 36.4% of bacteria, very good for 36.4% of bacteria, good for 22.7% of bacteria and intermediate for 4.5% of bacteria. Concerning the probability scores for exact identification of bacteria, they vary between 82% to 100% for Api 20 Strep and between 62.6% to 99.9% for Bis-Plus-D.

Conclusion: Api 20 Strep remains the best identification bacterial system and can be used by all bacteriology laboratories for good identification of bacterial species.