Association of Interleukin-6 and Interleukin -10 Gene Polymorphisms with Susceptibility and Clinical Features of Systemic Lupus Erythematosus in Bangladeshi Population

Author(s): Nasrin Nahar, Raisa Enayet Badhan, Sharmeen Ahmed, Rehana Razzak Khan, Shanjida Khondakar Setu

Background: Several cellular and cytokine abnormalities have been detected in Systemic lupus erythematosus (SLE) throughout the last three decades. Single nucleotide polymorphisms (SNPs) in cytokine genes have been shown to have an important role in the overall pathogenesis of SLE. Aim of the study was to demonstrate the association of IL-6 and IL-10 gene polymorphisms with susceptibility and clinical features in Bangladeshi SLE patients.

Methods: According to 2019 American College of Rheumatology (ACR)/ European League Against Rheumatism (EULAR) criteria, total 75 SLE patients and 75 healthy controls were enrolled in this study. IL-6 and IL-10 gene polymorphisms were detected by polymeric chain reaction- restriction fragment length polymorphism (PCR-RFLP) method. All statistical analysis was performed using the statistical pakage for social science (SPSS) program, 26 version.

Results: GG genotype and G allele of IL-6 (-174G/C) was higher in case compared to control group. The homozygous AA genotype, combined CA/AA genotype and A allele of IL-10 (-592C/A) were found significantly higher in patients than in control. The TC/CC and C allele of IL-10 (-819T/C) are found to be significant in patients than in control. TC/CC was found to be significantly higher in patients with oral ulcer as compared with patients having TT genotypes.

Conclusion: It can be concluded that IL-6 (-174G/C) and IL-10 (-592C/A), IL-10 (-819T/C) gene polymorphisms may play a role in SLE susceptibility in Bangladeshi population. The TC/CC genotype of IL-10 (-819T/C) gene appears to be associated with oral ulcer in SLE patients.