A flow Cytometry Gating Strategy for Profiling CD4+CD25+/highCD127-/low Regulatory T Cells in the Rheumatoid Arthritis Scenario

Author(s): Martínez Rosales Ricardo Ulises, Crespo Oliva Celia del Carmen, Chávez Valdés Sheila, Vazquez Arteaga Amalia, Coizeau Rodríguez Edelgis, Ávila Díaz Lismary, Freyre Corrales Giselle, Gonzalez Formental Hany Lianet, Chacón Quintero Yahima, Lemos Perez Gilda

Regulatory T cells (Tregs) has been consider as a potential biomarker in rheumatologic clinical trials as this cell population has been shown to be involved in the autoimmune diseases outcome. Human Tregs are defined as CD4+CD25+/highCD127-/low cells; however its accurate identification by flow cytometry has been argued, especially as a result of different gating strategies. A flow cytometry gating strategy was settled to measure human Tregs from PBMC isolated via density gradient using CPT tubes. With this method we performed a standardized procedure for Treg profiling, useful for immunomonitoring in clinicals assessment.